bas-ms phosphorimaging plates (FUJIFILM)
90
Structured Review
FUJIFILM
bas-ms phosphorimaging plates
Bas Ms Phosphorimaging Plates, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bas-ms+phosphorimaging+plates/las+3000/pm39987189-376-5-8
Average 90 stars, based on 1 article reviews
Bas Ms Phosphorimaging Plates, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bas-ms+phosphorimaging+plates/las+3000/pm39987189-376-5-8
Average 90 stars, based on 1 article reviews
bas-ms phosphorimaging plates - by Bioz Stars,
2026-10
90/100 stars
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Recombinant:Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Superscript III reverse transcriptase Thermo Fisher Scientific Cat# 18080044 RNase inhibitor ApexBio Cat# K1046 Monarch DNA Gel Extraction Kit New England Biolabs Cat# T1020L PureLink PCR Purification Kit Thermo Fisher Scientific Cat# K210003 KOD Hot Start DNA Polymerase EMD Millipore Cat# 71086-3 PrimeSTAR MAX DNA Polymerase Takara Cat# R045A Q5 High-Fidelity DNA polymerase New England Biolabs Cat# M0491L QIAprep Spin Miniprep Kit Qiagen Cat# 27106 Zymoprep Yeast Plasmid Miniprep II Kit Zymo Research Cat# D2004 PureLink HiPure Plasmid Miniprep Kit Thermo Fisher Scientific Cat# Article Title: Efficient genome replication in influenza A virus requires NS2 and sequence beyond the canonical promoter Article Snippet: Radioactive signals were collected using Article Title: Analysis of NS2-dependent effects on influenza PB1 segment extends replication requirements beyond the canonical promoter. Article Snippet: Radioactive signals were collected using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: After RNA extraction, approximately 400 mg of total RNA was reversed transcribed using 32P-labeled PA c/mRNA-specific primer (PA_135: 50-TGC TGC AAA TTT GTT TGT TCG-30), 32P-labeled PA vRNA-specific primer (PA_2119: 50-GGG TTT TGC TTA ATG CTTCTTGG-30), 32P-labeled 5S rRNA-specific primer (5S100: 50-TCCCAGGCGGTCTCCCATCC-30), and a SuperScript III reaction mix (Invitrogen) containing 1 U/mL RNase inhibitor (ApexBio). cDNA products derived from the viral RNAs were resolved using 7 M Urea/12%PAGE in 1x TBE as described previously.37 Radioactive signals were collected overnight using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: After RNA extraction, approximately 400 μg of total RNA was reversed transcribed using 32 P-labeled PA c/mRNA-specific primer (PA_135: 5′-TGC TGC AAA TTT GTT TGT TCG-3′), 32 P-labeled PA vRNA-specific primer (PA_2119: 5′-GGG TTT TGC TTA ATG CTT CTT GG-3′), 32 P-labeled 5S rRNA-specific primer (5S100: 5′-TCC CAG GCG GTC TCC CAT CC-3′), and a SuperScript III reaction mix (Invitrogen) containing 1 U/μL RNase inhibitor (ApexBio). Saline:Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Superscript III reverse transcriptase Thermo Fisher Scientific Cat# 18080044 RNase inhibitor ApexBio Cat# K1046 Monarch DNA Gel Extraction Kit New England Biolabs Cat# T1020L PureLink PCR Purification Kit Thermo Fisher Scientific Cat# K210003 KOD Hot Start DNA Polymerase EMD Millipore Cat# 71086-3 PrimeSTAR MAX DNA Polymerase Takara Cat# R045A Q5 High-Fidelity DNA polymerase New England Biolabs Cat# M0491L QIAprep Spin Miniprep Kit Qiagen Cat# 27106 Zymoprep Yeast Plasmid Miniprep II Kit Zymo Research Cat# D2004 PureLink HiPure Plasmid Miniprep Kit Thermo Fisher Scientific Cat# Article Title: Efficient genome replication in influenza A virus requires NS2 and sequence beyond the canonical promoter Article Snippet: Radioactive signals were collected using Article Title: Analysis of NS2-dependent effects on influenza PB1 segment extends replication requirements beyond the canonical promoter. Article Snippet: Radioactive signals were collected using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: After RNA extraction, approximately 400 mg of total RNA was reversed transcribed using 32P-labeled PA c/mRNA-specific primer (PA_135: 50-TGC TGC AAA TTT GTT TGT TCG-30), 32P-labeled PA vRNA-specific primer (PA_2119: 50-GGG TTT TGC TTA ATG CTTCTTGG-30), 32P-labeled 5S rRNA-specific primer (5S100: 50-TCCCAGGCGGTCTCCCATCC-30), and a SuperScript III reaction mix (Invitrogen) containing 1 U/mL RNase inhibitor (ApexBio). cDNA products derived from the viral RNAs were resolved using 7 M Urea/12%PAGE in 1x TBE as described previously.37 Radioactive signals were collected overnight using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: After RNA extraction, approximately 400 μg of total RNA was reversed transcribed using 32 P-labeled PA c/mRNA-specific primer (PA_135: 5′-TGC TGC AAA TTT GTT TGT TCG-3′), 32 P-labeled PA vRNA-specific primer (PA_2119: 5′-GGG TTT TGC TTA ATG CTT CTT GG-3′), 32 P-labeled 5S rRNA-specific primer (5S100: 5′-TCC CAG GCG GTC TCC CAT CC-3′), and a SuperScript III reaction mix (Invitrogen) containing 1 U/μL RNase inhibitor (ApexBio). Protease Inhibitor:Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Superscript III reverse transcriptase Thermo Fisher Scientific Cat# 18080044 RNase inhibitor ApexBio Cat# K1046 Monarch DNA Gel Extraction Kit New England Biolabs Cat# T1020L PureLink PCR Purification Kit Thermo Fisher Scientific Cat# K210003 KOD Hot Start DNA Polymerase EMD Millipore Cat# 71086-3 PrimeSTAR MAX DNA Polymerase Takara Cat# R045A Q5 High-Fidelity DNA polymerase New England Biolabs Cat# M0491L QIAprep Spin Miniprep Kit Qiagen Cat# 27106 Zymoprep Yeast Plasmid Miniprep II Kit Zymo Research Cat# D2004 PureLink HiPure Plasmid Miniprep Kit Thermo Fisher Scientific Cat# Article Title: Efficient genome replication in influenza A virus requires NS2 and sequence beyond the canonical promoter Article Snippet: Radioactive signals were collected using Article Title: Analysis of NS2-dependent effects on influenza PB1 segment extends replication requirements beyond the canonical promoter. Article Snippet: Radioactive signals were collected using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: After RNA extraction, approximately 400 mg of total RNA was reversed transcribed using 32P-labeled PA c/mRNA-specific primer (PA_135: 50-TGC TGC AAA TTT GTT TGT TCG-30), 32P-labeled PA vRNA-specific primer (PA_2119: 50-GGG TTT TGC TTA ATG CTTCTTGG-30), 32P-labeled 5S rRNA-specific primer (5S100: 50-TCCCAGGCGGTCTCCCATCC-30), and a SuperScript III reaction mix (Invitrogen) containing 1 U/mL RNase inhibitor (ApexBio). cDNA products derived from the viral RNAs were resolved using 7 M Urea/12%PAGE in 1x TBE as described previously.37 Radioactive signals were collected overnight using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: After RNA extraction, approximately 400 μg of total RNA was reversed transcribed using 32 P-labeled PA c/mRNA-specific primer (PA_135: 5′-TGC TGC AAA TTT GTT TGT TCG-3′), 32 P-labeled PA vRNA-specific primer (PA_2119: 5′-GGG TTT TGC TTA ATG CTT CTT GG-3′), 32 P-labeled 5S rRNA-specific primer (5S100: 5′-TCC CAG GCG GTC TCC CAT CC-3′), and a SuperScript III reaction mix (Invitrogen) containing 1 U/μL RNase inhibitor (ApexBio). SYBR Green Assay:Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Superscript III reverse transcriptase Thermo Fisher Scientific Cat# 18080044 RNase inhibitor ApexBio Cat# K1046 Monarch DNA Gel Extraction Kit New England Biolabs Cat# T1020L PureLink PCR Purification Kit Thermo Fisher Scientific Cat# K210003 KOD Hot Start DNA Polymerase EMD Millipore Cat# 71086-3 PrimeSTAR MAX DNA Polymerase Takara Cat# R045A Q5 High-Fidelity DNA polymerase New England Biolabs Cat# M0491L QIAprep Spin Miniprep Kit Qiagen Cat# 27106 Zymoprep Yeast Plasmid Miniprep II Kit Zymo Research Cat# D2004 PureLink HiPure Plasmid Miniprep Kit Thermo Fisher Scientific Cat# Article Title: Efficient genome replication in influenza A virus requires NS2 and sequence beyond the canonical promoter Article Snippet: Radioactive signals were collected using Article Title: Analysis of NS2-dependent effects on influenza PB1 segment extends replication requirements beyond the canonical promoter. Article Snippet: Radioactive signals were collected using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: After RNA extraction, approximately 400 mg of total RNA was reversed transcribed using 32P-labeled PA c/mRNA-specific primer (PA_135: 50-TGC TGC AAA TTT GTT TGT TCG-30), 32P-labeled PA vRNA-specific primer (PA_2119: 50-GGG TTT TGC TTA ATG CTTCTTGG-30), 32P-labeled 5S rRNA-specific primer (5S100: 50-TCCCAGGCGGTCTCCCATCC-30), and a SuperScript III reaction mix (Invitrogen) containing 1 U/mL RNase inhibitor (ApexBio). cDNA products derived from the viral RNAs were resolved using 7 M Urea/12%PAGE in 1x TBE as described previously.37 Radioactive signals were collected overnight using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: After RNA extraction, approximately 400 μg of total RNA was reversed transcribed using 32 P-labeled PA c/mRNA-specific primer (PA_135: 5′-TGC TGC AAA TTT GTT TGT TCG-3′), 32 P-labeled PA vRNA-specific primer (PA_2119: 5′-GGG TTT TGC TTA ATG CTT CTT GG-3′), 32 P-labeled 5S rRNA-specific primer (5S100: 5′-TCC CAG GCG GTC TCC CAT CC-3′), and a SuperScript III reaction mix (Invitrogen) containing 1 U/μL RNase inhibitor (ApexBio). Flow Cytometry:Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Superscript III reverse transcriptase Thermo Fisher Scientific Cat# 18080044 RNase inhibitor ApexBio Cat# K1046 Monarch DNA Gel Extraction Kit New England Biolabs Cat# T1020L PureLink PCR Purification Kit Thermo Fisher Scientific Cat# K210003 KOD Hot Start DNA Polymerase EMD Millipore Cat# 71086-3 PrimeSTAR MAX DNA Polymerase Takara Cat# R045A Q5 High-Fidelity DNA polymerase New England Biolabs Cat# M0491L QIAprep Spin Miniprep Kit Qiagen Cat# 27106 Zymoprep Yeast Plasmid Miniprep II Kit Zymo Research Cat# D2004 PureLink HiPure Plasmid Miniprep Kit Thermo Fisher Scientific Cat# Article Title: Efficient genome replication in influenza A virus requires NS2 and sequence beyond the canonical promoter Article Snippet: Radioactive signals were collected using Article Title: Analysis of NS2-dependent effects on influenza PB1 segment extends replication requirements beyond the canonical promoter. Article Snippet: Radioactive signals were collected using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: After RNA extraction, approximately 400 mg of total RNA was reversed transcribed using 32P-labeled PA c/mRNA-specific primer (PA_135: 50-TGC TGC AAA TTT GTT TGT TCG-30), 32P-labeled PA vRNA-specific primer (PA_2119: 50-GGG TTT TGC TTA ATG CTTCTTGG-30), 32P-labeled 5S rRNA-specific primer (5S100: 50-TCCCAGGCGGTCTCCCATCC-30), and a SuperScript III reaction mix (Invitrogen) containing 1 U/mL RNase inhibitor (ApexBio). cDNA products derived from the viral RNAs were resolved using 7 M Urea/12%PAGE in 1x TBE as described previously.37 Radioactive signals were collected overnight using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: After RNA extraction, approximately 400 μg of total RNA was reversed transcribed using 32 P-labeled PA c/mRNA-specific primer (PA_135: 5′-TGC TGC AAA TTT GTT TGT TCG-3′), 32 P-labeled PA vRNA-specific primer (PA_2119: 5′-GGG TTT TGC TTA ATG CTT CTT GG-3′), 32 P-labeled 5S rRNA-specific primer (5S100: 5′-TCC CAG GCG GTC TCC CAT CC-3′), and a SuperScript III reaction mix (Invitrogen) containing 1 U/μL RNase inhibitor (ApexBio). Reporter Assay:Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Superscript III reverse transcriptase Thermo Fisher Scientific Cat# 18080044 RNase inhibitor ApexBio Cat# K1046 Monarch DNA Gel Extraction Kit New England Biolabs Cat# T1020L PureLink PCR Purification Kit Thermo Fisher Scientific Cat# K210003 KOD Hot Start DNA Polymerase EMD Millipore Cat# 71086-3 PrimeSTAR MAX DNA Polymerase Takara Cat# R045A Q5 High-Fidelity DNA polymerase New England Biolabs Cat# M0491L QIAprep Spin Miniprep Kit Qiagen Cat# 27106 Zymoprep Yeast Plasmid Miniprep II Kit Zymo Research Cat# D2004 PureLink HiPure Plasmid Miniprep Kit Thermo Fisher Scientific Cat# Article Title: Efficient genome replication in influenza A virus requires NS2 and sequence beyond the canonical promoter Article Snippet: Radioactive signals were collected using Article Title: Analysis of NS2-dependent effects on influenza PB1 segment extends replication requirements beyond the canonical promoter. Article Snippet: Radioactive signals were collected using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: After RNA extraction, approximately 400 mg of total RNA was reversed transcribed using 32P-labeled PA c/mRNA-specific primer (PA_135: 50-TGC TGC AAA TTT GTT TGT TCG-30), 32P-labeled PA vRNA-specific primer (PA_2119: 50-GGG TTT TGC TTA ATG CTTCTTGG-30), 32P-labeled 5S rRNA-specific primer (5S100: 50-TCCCAGGCGGTCTCCCATCC-30), and a SuperScript III reaction mix (Invitrogen) containing 1 U/mL RNase inhibitor (ApexBio). cDNA products derived from the viral RNAs were resolved using 7 M Urea/12%PAGE in 1x TBE as described previously.37 Radioactive signals were collected overnight using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: After RNA extraction, approximately 400 μg of total RNA was reversed transcribed using 32 P-labeled PA c/mRNA-specific primer (PA_135: 5′-TGC TGC AAA TTT GTT TGT TCG-3′), 32 P-labeled PA vRNA-specific primer (PA_2119: 5′-GGG TTT TGC TTA ATG CTT CTT GG-3′), 32 P-labeled 5S rRNA-specific primer (5S100: 5′-TCC CAG GCG GTC TCC CAT CC-3′), and a SuperScript III reaction mix (Invitrogen) containing 1 U/μL RNase inhibitor (ApexBio). Western Blot:Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Superscript III reverse transcriptase Thermo Fisher Scientific Cat# 18080044 RNase inhibitor ApexBio Cat# K1046 Monarch DNA Gel Extraction Kit New England Biolabs Cat# T1020L PureLink PCR Purification Kit Thermo Fisher Scientific Cat# K210003 KOD Hot Start DNA Polymerase EMD Millipore Cat# 71086-3 PrimeSTAR MAX DNA Polymerase Takara Cat# R045A Q5 High-Fidelity DNA polymerase New England Biolabs Cat# M0491L QIAprep Spin Miniprep Kit Qiagen Cat# 27106 Zymoprep Yeast Plasmid Miniprep II Kit Zymo Research Cat# D2004 PureLink HiPure Plasmid Miniprep Kit Thermo Fisher Scientific Cat# Article Title: Efficient genome replication in influenza A virus requires NS2 and sequence beyond the canonical promoter Article Snippet: Radioactive signals were collected using Article Title: Analysis of NS2-dependent effects on influenza PB1 segment extends replication requirements beyond the canonical promoter. Article Snippet: Radioactive signals were collected using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: After RNA extraction, approximately 400 mg of total RNA was reversed transcribed using 32P-labeled PA c/mRNA-specific primer (PA_135: 50-TGC TGC AAA TTT GTT TGT TCG-30), 32P-labeled PA vRNA-specific primer (PA_2119: 50-GGG TTT TGC TTA ATG CTTCTTGG-30), 32P-labeled 5S rRNA-specific primer (5S100: 50-TCCCAGGCGGTCTCCCATCC-30), and a SuperScript III reaction mix (Invitrogen) containing 1 U/mL RNase inhibitor (ApexBio). cDNA products derived from the viral RNAs were resolved using 7 M Urea/12%PAGE in 1x TBE as described previously.37 Radioactive signals were collected overnight using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: After RNA extraction, approximately 400 μg of total RNA was reversed transcribed using 32 P-labeled PA c/mRNA-specific primer (PA_135: 5′-TGC TGC AAA TTT GTT TGT TCG-3′), 32 P-labeled PA vRNA-specific primer (PA_2119: 5′-GGG TTT TGC TTA ATG CTT CTT GG-3′), 32 P-labeled 5S rRNA-specific primer (5S100: 5′-TCC CAG GCG GTC TCC CAT CC-3′), and a SuperScript III reaction mix (Invitrogen) containing 1 U/μL RNase inhibitor (ApexBio). cDNA Synthesis:Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Superscript III reverse transcriptase Thermo Fisher Scientific Cat# 18080044 RNase inhibitor ApexBio Cat# K1046 Monarch DNA Gel Extraction Kit New England Biolabs Cat# T1020L PureLink PCR Purification Kit Thermo Fisher Scientific Cat# K210003 KOD Hot Start DNA Polymerase EMD Millipore Cat# 71086-3 PrimeSTAR MAX DNA Polymerase Takara Cat# R045A Q5 High-Fidelity DNA polymerase New England Biolabs Cat# M0491L QIAprep Spin Miniprep Kit Qiagen Cat# 27106 Zymoprep Yeast Plasmid Miniprep II Kit Zymo Research Cat# D2004 PureLink HiPure Plasmid Miniprep Kit Thermo Fisher Scientific Cat# Article Title: Efficient genome replication in influenza A virus requires NS2 and sequence beyond the canonical promoter Article Snippet: Radioactive signals were collected using Article Title: Analysis of NS2-dependent effects on influenza PB1 segment extends replication requirements beyond the canonical promoter. Article Snippet: Radioactive signals were collected using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: After RNA extraction, approximately 400 mg of total RNA was reversed transcribed using 32P-labeled PA c/mRNA-specific primer (PA_135: 50-TGC TGC AAA TTT GTT TGT TCG-30), 32P-labeled PA vRNA-specific primer (PA_2119: 50-GGG TTT TGC TTA ATG CTTCTTGG-30), 32P-labeled 5S rRNA-specific primer (5S100: 50-TCCCAGGCGGTCTCCCATCC-30), and a SuperScript III reaction mix (Invitrogen) containing 1 U/mL RNase inhibitor (ApexBio). cDNA products derived from the viral RNAs were resolved using 7 M Urea/12%PAGE in 1x TBE as described previously.37 Radioactive signals were collected overnight using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: After RNA extraction, approximately 400 μg of total RNA was reversed transcribed using 32 P-labeled PA c/mRNA-specific primer (PA_135: 5′-TGC TGC AAA TTT GTT TGT TCG-3′), 32 P-labeled PA vRNA-specific primer (PA_2119: 5′-GGG TTT TGC TTA ATG CTT CTT GG-3′), 32 P-labeled 5S rRNA-specific primer (5S100: 5′-TCC CAG GCG GTC TCC CAT CC-3′), and a SuperScript III reaction mix (Invitrogen) containing 1 U/μL RNase inhibitor (ApexBio). Reverse Transcription:Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Superscript III reverse transcriptase Thermo Fisher Scientific Cat# 18080044 RNase inhibitor ApexBio Cat# K1046 Monarch DNA Gel Extraction Kit New England Biolabs Cat# T1020L PureLink PCR Purification Kit Thermo Fisher Scientific Cat# K210003 KOD Hot Start DNA Polymerase EMD Millipore Cat# 71086-3 PrimeSTAR MAX DNA Polymerase Takara Cat# R045A Q5 High-Fidelity DNA polymerase New England Biolabs Cat# M0491L QIAprep Spin Miniprep Kit Qiagen Cat# 27106 Zymoprep Yeast Plasmid Miniprep II Kit Zymo Research Cat# D2004 PureLink HiPure Plasmid Miniprep Kit Thermo Fisher Scientific Cat# Article Title: Efficient genome replication in influenza A virus requires NS2 and sequence beyond the canonical promoter Article Snippet: Radioactive signals were collected using Article Title: Analysis of NS2-dependent effects on influenza PB1 segment extends replication requirements beyond the canonical promoter. Article Snippet: Radioactive signals were collected using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: After RNA extraction, approximately 400 mg of total RNA was reversed transcribed using 32P-labeled PA c/mRNA-specific primer (PA_135: 50-TGC TGC AAA TTT GTT TGT TCG-30), 32P-labeled PA vRNA-specific primer (PA_2119: 50-GGG TTT TGC TTA ATG CTTCTTGG-30), 32P-labeled 5S rRNA-specific primer (5S100: 50-TCCCAGGCGGTCTCCCATCC-30), and a SuperScript III reaction mix (Invitrogen) containing 1 U/mL RNase inhibitor (ApexBio). cDNA products derived from the viral RNAs were resolved using 7 M Urea/12%PAGE in 1x TBE as described previously.37 Radioactive signals were collected overnight using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: After RNA extraction, approximately 400 μg of total RNA was reversed transcribed using 32 P-labeled PA c/mRNA-specific primer (PA_135: 5′-TGC TGC AAA TTT GTT TGT TCG-3′), 32 P-labeled PA vRNA-specific primer (PA_2119: 5′-GGG TTT TGC TTA ATG CTT CTT GG-3′), 32 P-labeled 5S rRNA-specific primer (5S100: 5′-TCC CAG GCG GTC TCC CAT CC-3′), and a SuperScript III reaction mix (Invitrogen) containing 1 U/μL RNase inhibitor (ApexBio). Gel Extraction:Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Superscript III reverse transcriptase Thermo Fisher Scientific Cat# 18080044 RNase inhibitor ApexBio Cat# K1046 Monarch DNA Gel Extraction Kit New England Biolabs Cat# T1020L PureLink PCR Purification Kit Thermo Fisher Scientific Cat# K210003 KOD Hot Start DNA Polymerase EMD Millipore Cat# 71086-3 PrimeSTAR MAX DNA Polymerase Takara Cat# R045A Q5 High-Fidelity DNA polymerase New England Biolabs Cat# M0491L QIAprep Spin Miniprep Kit Qiagen Cat# 27106 Zymoprep Yeast Plasmid Miniprep II Kit Zymo Research Cat# D2004 PureLink HiPure Plasmid Miniprep Kit Thermo Fisher Scientific Cat# Article Title: Efficient genome replication in influenza A virus requires NS2 and sequence beyond the canonical promoter Article Snippet: Radioactive signals were collected using Article Title: Analysis of NS2-dependent effects on influenza PB1 segment extends replication requirements beyond the canonical promoter. Article Snippet: Radioactive signals were collected using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: After RNA extraction, approximately 400 mg of total RNA was reversed transcribed using 32P-labeled PA c/mRNA-specific primer (PA_135: 50-TGC TGC AAA TTT GTT TGT TCG-30), 32P-labeled PA vRNA-specific primer (PA_2119: 50-GGG TTT TGC TTA ATG CTTCTTGG-30), 32P-labeled 5S rRNA-specific primer (5S100: 50-TCCCAGGCGGTCTCCCATCC-30), and a SuperScript III reaction mix (Invitrogen) containing 1 U/mL RNase inhibitor (ApexBio). cDNA products derived from the viral RNAs were resolved using 7 M Urea/12%PAGE in 1x TBE as described previously.37 Radioactive signals were collected overnight using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: After RNA extraction, approximately 400 μg of total RNA was reversed transcribed using 32 P-labeled PA c/mRNA-specific primer (PA_135: 5′-TGC TGC AAA TTT GTT TGT TCG-3′), 32 P-labeled PA vRNA-specific primer (PA_2119: 5′-GGG TTT TGC TTA ATG CTT CTT GG-3′), 32 P-labeled 5S rRNA-specific primer (5S100: 5′-TCC CAG GCG GTC TCC CAT CC-3′), and a SuperScript III reaction mix (Invitrogen) containing 1 U/μL RNase inhibitor (ApexBio). Purification:Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Superscript III reverse transcriptase Thermo Fisher Scientific Cat# 18080044 RNase inhibitor ApexBio Cat# K1046 Monarch DNA Gel Extraction Kit New England Biolabs Cat# T1020L PureLink PCR Purification Kit Thermo Fisher Scientific Cat# K210003 KOD Hot Start DNA Polymerase EMD Millipore Cat# 71086-3 PrimeSTAR MAX DNA Polymerase Takara Cat# R045A Q5 High-Fidelity DNA polymerase New England Biolabs Cat# M0491L QIAprep Spin Miniprep Kit Qiagen Cat# 27106 Zymoprep Yeast Plasmid Miniprep II Kit Zymo Research Cat# D2004 PureLink HiPure Plasmid Miniprep Kit Thermo Fisher Scientific Cat# Article Title: Efficient genome replication in influenza A virus requires NS2 and sequence beyond the canonical promoter Article Snippet: Radioactive signals were collected using Article Title: Analysis of NS2-dependent effects on influenza PB1 segment extends replication requirements beyond the canonical promoter. Article Snippet: Radioactive signals were collected using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: After RNA extraction, approximately 400 mg of total RNA was reversed transcribed using 32P-labeled PA c/mRNA-specific primer (PA_135: 50-TGC TGC AAA TTT GTT TGT TCG-30), 32P-labeled PA vRNA-specific primer (PA_2119: 50-GGG TTT TGC TTA ATG CTTCTTGG-30), 32P-labeled 5S rRNA-specific primer (5S100: 50-TCCCAGGCGGTCTCCCATCC-30), and a SuperScript III reaction mix (Invitrogen) containing 1 U/mL RNase inhibitor (ApexBio). cDNA products derived from the viral RNAs were resolved using 7 M Urea/12%PAGE in 1x TBE as described previously.37 Radioactive signals were collected overnight using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: After RNA extraction, approximately 400 μg of total RNA was reversed transcribed using 32 P-labeled PA c/mRNA-specific primer (PA_135: 5′-TGC TGC AAA TTT GTT TGT TCG-3′), 32 P-labeled PA vRNA-specific primer (PA_2119: 5′-GGG TTT TGC TTA ATG CTT CTT GG-3′), 32 P-labeled 5S rRNA-specific primer (5S100: 5′-TCC CAG GCG GTC TCC CAT CC-3′), and a SuperScript III reaction mix (Invitrogen) containing 1 U/μL RNase inhibitor (ApexBio). Plasmid Preparation:Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Superscript III reverse transcriptase Thermo Fisher Scientific Cat# 18080044 RNase inhibitor ApexBio Cat# K1046 Monarch DNA Gel Extraction Kit New England Biolabs Cat# T1020L PureLink PCR Purification Kit Thermo Fisher Scientific Cat# K210003 KOD Hot Start DNA Polymerase EMD Millipore Cat# 71086-3 PrimeSTAR MAX DNA Polymerase Takara Cat# R045A Q5 High-Fidelity DNA polymerase New England Biolabs Cat# M0491L QIAprep Spin Miniprep Kit Qiagen Cat# 27106 Zymoprep Yeast Plasmid Miniprep II Kit Zymo Research Cat# D2004 PureLink HiPure Plasmid Miniprep Kit Thermo Fisher Scientific Cat# Article Title: Efficient genome replication in influenza A virus requires NS2 and sequence beyond the canonical promoter Article Snippet: Radioactive signals were collected using Article Title: Analysis of NS2-dependent effects on influenza PB1 segment extends replication requirements beyond the canonical promoter. Article Snippet: Radioactive signals were collected using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: After RNA extraction, approximately 400 mg of total RNA was reversed transcribed using 32P-labeled PA c/mRNA-specific primer (PA_135: 50-TGC TGC AAA TTT GTT TGT TCG-30), 32P-labeled PA vRNA-specific primer (PA_2119: 50-GGG TTT TGC TTA ATG CTTCTTGG-30), 32P-labeled 5S rRNA-specific primer (5S100: 50-TCCCAGGCGGTCTCCCATCC-30), and a SuperScript III reaction mix (Invitrogen) containing 1 U/mL RNase inhibitor (ApexBio). cDNA products derived from the viral RNAs were resolved using 7 M Urea/12%PAGE in 1x TBE as described previously.37 Radioactive signals were collected overnight using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: After RNA extraction, approximately 400 μg of total RNA was reversed transcribed using 32 P-labeled PA c/mRNA-specific primer (PA_135: 5′-TGC TGC AAA TTT GTT TGT TCG-3′), 32 P-labeled PA vRNA-specific primer (PA_2119: 5′-GGG TTT TGC TTA ATG CTT CTT GG-3′), 32 P-labeled 5S rRNA-specific primer (5S100: 5′-TCC CAG GCG GTC TCC CAT CC-3′), and a SuperScript III reaction mix (Invitrogen) containing 1 U/μL RNase inhibitor (ApexBio). Sequencing:Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Superscript III reverse transcriptase Thermo Fisher Scientific Cat# 18080044 RNase inhibitor ApexBio Cat# K1046 Monarch DNA Gel Extraction Kit New England Biolabs Cat# T1020L PureLink PCR Purification Kit Thermo Fisher Scientific Cat# K210003 KOD Hot Start DNA Polymerase EMD Millipore Cat# 71086-3 PrimeSTAR MAX DNA Polymerase Takara Cat# R045A Q5 High-Fidelity DNA polymerase New England Biolabs Cat# M0491L QIAprep Spin Miniprep Kit Qiagen Cat# 27106 Zymoprep Yeast Plasmid Miniprep II Kit Zymo Research Cat# D2004 PureLink HiPure Plasmid Miniprep Kit Thermo Fisher Scientific Cat# Article Title: Efficient genome replication in influenza A virus requires NS2 and sequence beyond the canonical promoter Article Snippet: Radioactive signals were collected using Article Title: Analysis of NS2-dependent effects on influenza PB1 segment extends replication requirements beyond the canonical promoter. Article Snippet: Radioactive signals were collected using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: After RNA extraction, approximately 400 mg of total RNA was reversed transcribed using 32P-labeled PA c/mRNA-specific primer (PA_135: 50-TGC TGC AAA TTT GTT TGT TCG-30), 32P-labeled PA vRNA-specific primer (PA_2119: 50-GGG TTT TGC TTA ATG CTTCTTGG-30), 32P-labeled 5S rRNA-specific primer (5S100: 50-TCCCAGGCGGTCTCCCATCC-30), and a SuperScript III reaction mix (Invitrogen) containing 1 U/mL RNase inhibitor (ApexBio). cDNA products derived from the viral RNAs were resolved using 7 M Urea/12%PAGE in 1x TBE as described previously.37 Radioactive signals were collected overnight using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: After RNA extraction, approximately 400 μg of total RNA was reversed transcribed using 32 P-labeled PA c/mRNA-specific primer (PA_135: 5′-TGC TGC AAA TTT GTT TGT TCG-3′), 32 P-labeled PA vRNA-specific primer (PA_2119: 5′-GGG TTT TGC TTA ATG CTT CTT GG-3′), 32 P-labeled 5S rRNA-specific primer (5S100: 5′-TCC CAG GCG GTC TCC CAT CC-3′), and a SuperScript III reaction mix (Invitrogen) containing 1 U/μL RNase inhibitor (ApexBio). Activity Assay:Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Superscript III reverse transcriptase Thermo Fisher Scientific Cat# 18080044 RNase inhibitor ApexBio Cat# K1046 Monarch DNA Gel Extraction Kit New England Biolabs Cat# T1020L PureLink PCR Purification Kit Thermo Fisher Scientific Cat# K210003 KOD Hot Start DNA Polymerase EMD Millipore Cat# 71086-3 PrimeSTAR MAX DNA Polymerase Takara Cat# R045A Q5 High-Fidelity DNA polymerase New England Biolabs Cat# M0491L QIAprep Spin Miniprep Kit Qiagen Cat# 27106 Zymoprep Yeast Plasmid Miniprep II Kit Zymo Research Cat# D2004 PureLink HiPure Plasmid Miniprep Kit Thermo Fisher Scientific Cat# Article Title: Efficient genome replication in influenza A virus requires NS2 and sequence beyond the canonical promoter Article Snippet: Radioactive signals were collected using Article Title: Analysis of NS2-dependent effects on influenza PB1 segment extends replication requirements beyond the canonical promoter. Article Snippet: Radioactive signals were collected using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: After RNA extraction, approximately 400 mg of total RNA was reversed transcribed using 32P-labeled PA c/mRNA-specific primer (PA_135: 50-TGC TGC AAA TTT GTT TGT TCG-30), 32P-labeled PA vRNA-specific primer (PA_2119: 50-GGG TTT TGC TTA ATG CTTCTTGG-30), 32P-labeled 5S rRNA-specific primer (5S100: 50-TCCCAGGCGGTCTCCCATCC-30), and a SuperScript III reaction mix (Invitrogen) containing 1 U/mL RNase inhibitor (ApexBio). cDNA products derived from the viral RNAs were resolved using 7 M Urea/12%PAGE in 1x TBE as described previously.37 Radioactive signals were collected overnight using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: After RNA extraction, approximately 400 μg of total RNA was reversed transcribed using 32 P-labeled PA c/mRNA-specific primer (PA_135: 5′-TGC TGC AAA TTT GTT TGT TCG-3′), 32 P-labeled PA vRNA-specific primer (PA_2119: 5′-GGG TTT TGC TTA ATG CTT CTT GG-3′), 32 P-labeled 5S rRNA-specific primer (5S100: 5′-TCC CAG GCG GTC TCC CAT CC-3′), and a SuperScript III reaction mix (Invitrogen) containing 1 U/μL RNase inhibitor (ApexBio). Software:Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Superscript III reverse transcriptase Thermo Fisher Scientific Cat# 18080044 RNase inhibitor ApexBio Cat# K1046 Monarch DNA Gel Extraction Kit New England Biolabs Cat# T1020L PureLink PCR Purification Kit Thermo Fisher Scientific Cat# K210003 KOD Hot Start DNA Polymerase EMD Millipore Cat# 71086-3 PrimeSTAR MAX DNA Polymerase Takara Cat# R045A Q5 High-Fidelity DNA polymerase New England Biolabs Cat# M0491L QIAprep Spin Miniprep Kit Qiagen Cat# 27106 Zymoprep Yeast Plasmid Miniprep II Kit Zymo Research Cat# D2004 PureLink HiPure Plasmid Miniprep Kit Thermo Fisher Scientific Cat# Article Title: Efficient genome replication in influenza A virus requires NS2 and sequence beyond the canonical promoter Article Snippet: Radioactive signals were collected using Article Title: Analysis of NS2-dependent effects on influenza PB1 segment extends replication requirements beyond the canonical promoter. Article Snippet: Radioactive signals were collected using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: After RNA extraction, approximately 400 mg of total RNA was reversed transcribed using 32P-labeled PA c/mRNA-specific primer (PA_135: 50-TGC TGC AAA TTT GTT TGT TCG-30), 32P-labeled PA vRNA-specific primer (PA_2119: 50-GGG TTT TGC TTA ATG CTTCTTGG-30), 32P-labeled 5S rRNA-specific primer (5S100: 50-TCCCAGGCGGTCTCCCATCC-30), and a SuperScript III reaction mix (Invitrogen) containing 1 U/mL RNase inhibitor (ApexBio). cDNA products derived from the viral RNAs were resolved using 7 M Urea/12%PAGE in 1x TBE as described previously.37 Radioactive signals were collected overnight using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: After RNA extraction, approximately 400 μg of total RNA was reversed transcribed using 32 P-labeled PA c/mRNA-specific primer (PA_135: 5′-TGC TGC AAA TTT GTT TGT TCG-3′), 32 P-labeled PA vRNA-specific primer (PA_2119: 5′-GGG TTT TGC TTA ATG CTT CTT GG-3′), 32 P-labeled 5S rRNA-specific primer (5S100: 5′-TCC CAG GCG GTC TCC CAT CC-3′), and a SuperScript III reaction mix (Invitrogen) containing 1 U/μL RNase inhibitor (ApexBio). Polymerase Chain Reaction:Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Superscript III reverse transcriptase Thermo Fisher Scientific Cat# 18080044 RNase inhibitor ApexBio Cat# K1046 Monarch DNA Gel Extraction Kit New England Biolabs Cat# T1020L PureLink PCR Purification Kit Thermo Fisher Scientific Cat# K210003 KOD Hot Start DNA Polymerase EMD Millipore Cat# 71086-3 PrimeSTAR MAX DNA Polymerase Takara Cat# R045A Q5 High-Fidelity DNA polymerase New England Biolabs Cat# M0491L QIAprep Spin Miniprep Kit Qiagen Cat# 27106 Zymoprep Yeast Plasmid Miniprep II Kit Zymo Research Cat# D2004 PureLink HiPure Plasmid Miniprep Kit Thermo Fisher Scientific Cat# Article Title: Efficient genome replication in influenza A virus requires NS2 and sequence beyond the canonical promoter Article Snippet: Radioactive signals were collected using Article Title: Analysis of NS2-dependent effects on influenza PB1 segment extends replication requirements beyond the canonical promoter. Article Snippet: Radioactive signals were collected using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: After RNA extraction, approximately 400 mg of total RNA was reversed transcribed using 32P-labeled PA c/mRNA-specific primer (PA_135: 50-TGC TGC AAA TTT GTT TGT TCG-30), 32P-labeled PA vRNA-specific primer (PA_2119: 50-GGG TTT TGC TTA ATG CTTCTTGG-30), 32P-labeled 5S rRNA-specific primer (5S100: 50-TCCCAGGCGGTCTCCCATCC-30), and a SuperScript III reaction mix (Invitrogen) containing 1 U/mL RNase inhibitor (ApexBio). cDNA products derived from the viral RNAs were resolved using 7 M Urea/12%PAGE in 1x TBE as described previously.37 Radioactive signals were collected overnight using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: After RNA extraction, approximately 400 μg of total RNA was reversed transcribed using 32 P-labeled PA c/mRNA-specific primer (PA_135: 5′-TGC TGC AAA TTT GTT TGT TCG-3′), 32 P-labeled PA vRNA-specific primer (PA_2119: 5′-GGG TTT TGC TTA ATG CTT CTT GG-3′), 32 P-labeled 5S rRNA-specific primer (5S100: 5′-TCC CAG GCG GTC TCC CAT CC-3′), and a SuperScript III reaction mix (Invitrogen) containing 1 U/μL RNase inhibitor (ApexBio). RNA Extraction:Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Superscript III reverse transcriptase Thermo Fisher Scientific Cat# 18080044 RNase inhibitor ApexBio Cat# K1046 Monarch DNA Gel Extraction Kit New England Biolabs Cat# T1020L PureLink PCR Purification Kit Thermo Fisher Scientific Cat# K210003 KOD Hot Start DNA Polymerase EMD Millipore Cat# 71086-3 PrimeSTAR MAX DNA Polymerase Takara Cat# R045A Q5 High-Fidelity DNA polymerase New England Biolabs Cat# M0491L QIAprep Spin Miniprep Kit Qiagen Cat# 27106 Zymoprep Yeast Plasmid Miniprep II Kit Zymo Research Cat# D2004 PureLink HiPure Plasmid Miniprep Kit Thermo Fisher Scientific Cat# Article Title: Efficient genome replication in influenza A virus requires NS2 and sequence beyond the canonical promoter Article Snippet: Radioactive signals were collected using Article Title: Analysis of NS2-dependent effects on influenza PB1 segment extends replication requirements beyond the canonical promoter. Article Snippet: Radioactive signals were collected using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: After RNA extraction, approximately 400 mg of total RNA was reversed transcribed using 32P-labeled PA c/mRNA-specific primer (PA_135: 50-TGC TGC AAA TTT GTT TGT TCG-30), 32P-labeled PA vRNA-specific primer (PA_2119: 50-GGG TTT TGC TTA ATG CTTCTTGG-30), 32P-labeled 5S rRNA-specific primer (5S100: 50-TCCCAGGCGGTCTCCCATCC-30), and a SuperScript III reaction mix (Invitrogen) containing 1 U/mL RNase inhibitor (ApexBio). cDNA products derived from the viral RNAs were resolved using 7 M Urea/12%PAGE in 1x TBE as described previously.37 Radioactive signals were collected overnight using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: After RNA extraction, approximately 400 μg of total RNA was reversed transcribed using 32 P-labeled PA c/mRNA-specific primer (PA_135: 5′-TGC TGC AAA TTT GTT TGT TCG-3′), 32 P-labeled PA vRNA-specific primer (PA_2119: 5′-GGG TTT TGC TTA ATG CTT CTT GG-3′), 32 P-labeled 5S rRNA-specific primer (5S100: 5′-TCC CAG GCG GTC TCC CAT CC-3′), and a SuperScript III reaction mix (Invitrogen) containing 1 U/μL RNase inhibitor (ApexBio). Derivative Assay:Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Superscript III reverse transcriptase Thermo Fisher Scientific Cat# 18080044 RNase inhibitor ApexBio Cat# K1046 Monarch DNA Gel Extraction Kit New England Biolabs Cat# T1020L PureLink PCR Purification Kit Thermo Fisher Scientific Cat# K210003 KOD Hot Start DNA Polymerase EMD Millipore Cat# 71086-3 PrimeSTAR MAX DNA Polymerase Takara Cat# R045A Q5 High-Fidelity DNA polymerase New England Biolabs Cat# M0491L QIAprep Spin Miniprep Kit Qiagen Cat# 27106 Zymoprep Yeast Plasmid Miniprep II Kit Zymo Research Cat# D2004 PureLink HiPure Plasmid Miniprep Kit Thermo Fisher Scientific Cat# Article Title: Efficient genome replication in influenza A virus requires NS2 and sequence beyond the canonical promoter Article Snippet: Radioactive signals were collected using Article Title: Analysis of NS2-dependent effects on influenza PB1 segment extends replication requirements beyond the canonical promoter. Article Snippet: Radioactive signals were collected using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning. Article Snippet: After RNA extraction, approximately 400 mg of total RNA was reversed transcribed using 32P-labeled PA c/mRNA-specific primer (PA_135: 50-TGC TGC AAA TTT GTT TGT TCG-30), 32P-labeled PA vRNA-specific primer (PA_2119: 50-GGG TTT TGC TTA ATG CTTCTTGG-30), 32P-labeled 5S rRNA-specific primer (5S100: 50-TCCCAGGCGGTCTCCCATCC-30), and a SuperScript III reaction mix (Invitrogen) containing 1 U/mL RNase inhibitor (ApexBio). cDNA products derived from the viral RNAs were resolved using 7 M Urea/12%PAGE in 1x TBE as described previously.37 Radioactive signals were collected overnight using Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: Article Title: Probing the functional constraints of influenza A virus NEP by deep mutational scanning Article Snippet: After RNA extraction, approximately 400 μg of total RNA was reversed transcribed using 32 P-labeled PA c/mRNA-specific primer (PA_135: 5′-TGC TGC AAA TTT GTT TGT TCG-3′), 32 P-labeled PA vRNA-specific primer (PA_2119: 5′-GGG TTT TGC TTA ATG CTT CTT GG-3′), 32 P-labeled 5S rRNA-specific primer (5S100: 5′-TCC CAG GCG GTC TCC CAT CC-3′), and a SuperScript III reaction mix (Invitrogen) containing 1 U/μL RNase inhibitor (ApexBio). |